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KMID : 1094720120170010185
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Biotechnology and Bioprocess Engineering
2012 Volume.17 No. 1 p.185 ~ p.194
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Preparation of peptide-loaded polymer microparticles using supercritical carbon dioxide
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Jung In-Il
Haam Seoung-Joo
Lim Gio-Bin
Ryu Jong-Hoon
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Abstract
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In this study, peptide-loaded microparticles were prepared using an aerosol solvent extraction system (ASES) by employing supercritical carbon dioxide as an antisolvent. The effects of the molecular weight of poly(Llactide) (PLLA), poly(ethylene glycol) (PEG), the block length of methoxy poly(ethylene glycol)-b-poly(L-lactide) (mPEG-PLLA), the blending of PLLA and PEG, and the drug-to-polymer feed ratio on the formation of leuprolide acetate (LA)-loaded microparticles and their release characteristics were investigated. Scanning electron microscope observations showed that the LA-loaded polymer particles had a spherical morphology with a smooth surface. The entrapment efficiency of LA in the ASES-processed microparticles was found to be extremely high (about 99%), whereas the initial release rate of the LA-loaded microparticles was very low for PLLA. The release rate of LA was observed to increase as the PEG block length of mPEG-PLLA and/or the drug content in the microparticles increased. When PLLA was blended with PEG, the release rate of LA from the PLLA/PEG microparticles was significantly faster compared with the corresponding mPEG-PLLA copolymer.
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KEYWORD
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ASES, supercritical fluid, leuprolide acetate, PLLA, microparticles
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